Human NLRP2/NALP2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4684
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human NLRP2/NALP2
Met1-Thr208
Accession # Q9NX02
Met1-Thr208
Accession # Q9NX02
Specificity
Detects human NLRP2/NALP2 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human NLRP2/NALP2 Antibody
Detection of Human NLRP2/NALP2 by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Human NLRP2/NALP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4684) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NLRP2/NALP2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human NLRP2/NALP2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: A549 human lung carcinoma cell line and LNCaP human prostate cancer cell line
Sample: A549 human lung carcinoma cell line and LNCaP human prostate cancer cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NLRP2/NALP2
NALP2 (NAcht Leucine-rich repeat Protein 2, also known as NLRP2 and PAN1) is a cytosolic, 121 kDa member of the NLRP family of proteins. It is expressed in macrophages and exhibits divergent effects on inflammation. Via NF kappaB, it suppresses TNF-alpha production in response to LPS; via procaspase-1, it promotes proIL-1 beta cleavage and release. Human NALP2 is 1062 amino acids (aa) in length. It contains an N-terminal Pyrin domain (aa 9‑90), followed by a Nacht region and nine LRRs (aa 467‑1033). Four alternate isoforms exist. Three show deletions of aa’s 109‑132, 133‑154 and 847‑1062, respectively; a fourth shows a 38 aa substitution for aa’s 1‑903. No close rodent counterparts have been reported.
Long Name
NLR Family, Pyrin Domain Containing 2
Alternate Names
NALP2, NBS1, PAN1, PYPAF2
Gene Symbol
NLRP2
UniProt
Additional NLRP2/NALP2 Products
Product Documents for Human NLRP2/NALP2 Antibody
Product Specific Notices for Human NLRP2/NALP2 Antibody
For research use only
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