Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human NLRP6/NALP6 PE-conjugated Monoclonal Antibody (Catalog # IC9145P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human NLRP6/NALP6 PE-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: Jurkat human acute T cell leukemia cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: NLRP6/NALP6

NLRP6 (Nod-Like Receptor Pyrin domain-containing protein 6), also known as NALP6, PYPAF5 and PAN3, is an 80-85 kDa cytoplasmic member of the NLRP family of proteins. Human NLRP is 892 amino acids (aa) in length, and contains an N-terminal Pyrin domain (aa 1-103), one Nacht region (aa 197-350), and five consecutive C-terminqal leucine-rich repeats (aa 462-868). It is expressed in select cell types, including eosinophils, neutrophils, CD4+ and CD8+ T cells, neurons, myofibroblasts, and intestinal goblet cells plus columnar epithelium. NLRP6 is perhaps best known for its participation in the formation of inflammasomes. Inflammasomes are oligomeric intracellular complexes that serve as detectors for "danger signals" that appear following microbial invasion or unexpected cell death. Following detection, recruited components to the complex (such as Caspase-1) are activated, and subsequently themselves serve to activate pro-IL-1 beta and IL-18. The actual detection of these signals is mediated by the NLRP family, possibly in a tissue specific manner. NLRP6 has been identified in both damaged peripheral nerve and intestinal epithelium. In goblet cells, NLRP6 drives mucus secretion; in intestinal columnar epithelium, NLRP6 acts as a homeostatic agent that promotes epithelial barrier integrity. It accomplishes this by inducing mature IL-18 secretion, a step that then reduces IL-22BP production and leads to an increase in IL-22, a cytokine known for its pro-mucosal homeostatic properties. Although most insults to the intestinal mucosa likely arise from bacteria, NLRP6 is also known to recognize dsRNA-based viruses such as norovirus, and induce the expression of antiviral cytokines such as IFN-alpha, -beta and -lambda. Over aa 35-193, human NLRP6 shares 69% aa sequence identity with mouse NLRP6.

Long Name

NLR Family, Pyrin Domain Containing 6

Alternate Names

AVR, CLR11.4, NALP6, NAVR, PAN3, PYPAF5

Entrez Gene IDs

171389 (Human); 101613 (Mouse); 171390 (Rat)

Gene Symbol

NLRP6

UniProt

P59044

Additional NLRP6/NALP6 Products

Product Documents for Human NLRP6/NALP6 PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human NLRP6/NALP6 PE-conjugated Antibody

For research use only

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Flow Cytometry

Human NLRP6/NALP6 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC9145P

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all NLRP6/NALP6 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human NLRP6/NALP6 PE-conjugated Antibody

Detection of NLRP6/NALP6 in Jurkat Human Cell Line by Flow Cytometry.