Human NM23-H1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6256
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 642817
Product Summary for Human NM23-H1 Antibody
Immunogen
E. coli-derived recombinant human NM23‑H1
Met1-Glu152
Accession # P15531
Met1-Glu152
Accession # P15531
Specificity
Detects human NM23‑H1 in Western blots. In Western blots, no cross-reactivity with recombinant human NM23-H2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human NM23-H1 Antibody
Detection of Human NM23‑H1 by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human NM23-H1 Monoclonal Antibody (Catalog # MAB6256) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human NM23-H1 and recombinant human NM23-H2 (10 ng/lane) were included. A specific band was detected for NM23-H1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human NM23-H1 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: A431 human epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and K562 human chronic myelogenous leukemia cell line
Sample: A431 human epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and K562 human chronic myelogenous leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NM23-H1
NM23-H1 (Non-metastatic protein 23 homolog 1; also NDKA) is a 19‑20 kDa member of the NDK family of enzymes. NM23-H1 is ubiquitous in expression and performs multiple functions. It forms disulfide-linked homohexamers, and heterohexamers with NM23-H2, generating a nucleoside diphosphate kinase that catalyzes a phosphoryl transfer from ATP to a nucleoside diphosphate. It also shows His and Ser/Thr protein kinase activity and forms covalent linkages with molecules diverse as p53 and STRAP. It is found both intracellularly and in blood at ng/mL concentrations. Human NM23-H1 is 152 amino acids (aa) in length, contains one NDP kinase domain (aa 5‑134), and shows acetylation at Ala2 and Lys56, plus phosphorylation at Tyr52, Thr94, Ser122, and Ser125. Human NM23-H1 shares 89% aa identity with human 17‑18 kDa NM23-H2 and 94% aa identity with mouse NM23-H1. A second H1 isoform named NM23-H1B with 25 additional aa at the N‑terminus has also been described.
Long Name
Non-metastatic Protein 23 Homolog 1
Alternate Names
GAAD, NDKA, NDPKA, NM23A, NM23H1, NME1
Entrez Gene IDs
4830 (Human)
Gene Symbol
NME1
UniProt
Additional NM23-H1 Products
Product Documents for Human NM23-H1 Antibody
Product Specific Notices for Human NM23-H1 Antibody
For research use only
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