Human NOD1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7090
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 626919
Product Specifications
Immunogen
E. coli-derived recombinant human NOD1
Lys830-Phe953
Accession # Q9Y239
Lys830-Phe953
Accession # Q9Y239
Specificity
Detects human NOD1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human NOD2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human NOD1 Antibody
Detection of Human NOD1 by Western Blot.
Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human NOD1 or human NOD2, HT-29 human colon adenocarcinoma cell line, and SW480 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NOD1 Monoclonal Antibody (Catalog # MAB7090) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NOD1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
NOD1 in Human Spleen.
NOD1 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human NOD1 Monoclonal Antibody (Catalog # MAB7090) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human NOD1 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen
Sample: Immersion fixed paraffin-embedded sections of human spleen
Western Blot
1 µg/mL
Sample: HEK293T human embryonic kidney cell line transfected with human NOD1 or human NOD2, HT‑29 human colon adenocarcinoma cell line, and SW480 human colorectal adenocarcinoma cell line
Sample: HEK293T human embryonic kidney cell line transfected with human NOD1 or human NOD2, HT‑29 human colon adenocarcinoma cell line, and SW480 human colorectal adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NOD1
Long Name
Nucleotide-binding Oligomerization Domain-containing Protein 1
Alternate Names
CARD4, CLR7.1, NLRC1
Gene Symbol
NOD1
UniProt
Additional NOD1 Products
Product Documents for Human NOD1 Antibody
Product Specific Notices for Human NOD1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...