Human Notch-3 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6870P

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 603532

View all Notch-3 Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Notch-3
Ala40-Glu467
Accession # Q9UM47

Specificity

Detects human Notch-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Notch-3 ICD (aa 2195-2321), rhNotch-1, rhNotch-1 ICD (aa 2251-2556), rhNotch-2, rhNotch-2 ICD (aa 2063-2413), rhNotch-4, rhNotch-4 ICD (aa 1778-2003), recombinant mouse Notch-3, or recombinant rat DLL1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Notch-3 PE-conjugated Antibody

Detection of Notch-3 antibody in BG01V Human Stem Cells antibody by Flow Cytometry.

Detection of Notch-3 in BG01V Human Stem Cells by Flow Cytometry.

BG01V human embryonic stem cells were stained with Mouse Anti-Human Notch-3 PE-conjugated Monoclonal Antibody (Catalog # FAB6870P, filled histogram) or isotype control antibody (IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of Notch-3 in SUP-T1 Human Cell Line by Flow Cytometry

SUP-T1 human T cell lymphoblastic lymphoma cell line was stained with Mouse Anti-Human Notch-3 PE-conjugated Monoclonal Antibody (Catalog # FAB6870P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Notch-3 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: BG01V human embryonic stem cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Notch-3

Human Notch-3 is a member of the Notch family of type I transmembrane glycoproteins involved in early-event developmental processes. The 2321 amino acid (aa) Notch-3 precursor contains a 1603 aa extracellular region with 34 EGF-like repeats. Repeats 1-11 of human Notch-3 are within the sequence used as an immunogen, and share 94% aa identity with mouse and rat Notch-3. Repeats 11 and 12 are critical for binding the ligands Jagged and Delta. Notch-3 is expressed in vascular smooth muscle, proliferating neuroepithelium, CD4-CD8- thymocytes, regulatory T cells and T-ALL leukemia cells. Mutations in the first 5 EGF repeats of Notch-3 in humans can cause CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy).

Alternate Names

Notch3

Entrez Gene IDs

4854 (Human); 18131 (Mouse)

Gene Symbol

NOTCH3

UniProt

Q9UM47

Additional Notch-3 Products

Product Documents for Human Notch-3 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Notch-3 PE-conjugated Antibody

For research use only

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