Human Notum Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9356

R&D Systems, part of Bio-Techne
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MAB9356-100
MAB9356-SP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 962216

View all Notum Antibodies »

Product Summary for Human Notum Antibody

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Notum
Ser81-Thr451
Accession # Q6P988

Specificity

Detects human Notum in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Notum Antibody

Detection of Human Notum antibody by Western Blot.

Detection of Human Notum by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Notum Monoclonal Antibody (Catalog # MAB9356) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Notum at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Notum Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Notum

Notum is an evolutionarily conserved 60 kDa secreted deacylase that regulates Wnt activity (1, 2). It is tethered to the cell surface by binding to Glypican-like sulfated proteoglycans, and it functions as a Glypican-dependent Wnt inhibitor (1-4). Notum hydrolyzes the palmitoyl moiety from Wnt-3a, making the Wnt more hydrophilic and unable to bind to the Frizzled-8 receptor (1, 2). It also induces the release of cell surface GPI-anchored proteins such as Glypican-3, Cadherin-13, and uPAR (4). Notum regulates head regeneration in planaria (3), head formation and neural induction in Xenopus (1), and long range activity of hedgehog proteins in Drosophila (5). It is up-regulated in some human hepatocellular carcinomas and colorectal cancers (6, 7). Within amino acids
81-451, human Notum shares 92% aa sequence identity with mouse and rat Notum.

References

  1. Zhang, X. et al. (2015) Dev. Cell 32:719.
  2. Kakugawa, S. et al. (2015) Nature 519:187.
  3. Petersen, C.P. and P.W. Reddien (2011) Science 332:852.
  4. Traister, A. et al. (2008) Biochem. J. 410:503.
  5. Ayers, K.L. et al. (2010) Dev. Cell 18:605.
  6. Torisu, Y. et al. (2008) Cancer Sci. 99:1139.
  7. De Robertis, M. et al. (2015) Oncotarget 6:41237.

Alternate Names

EC 3.-, notum pectinacetylesterase homolog (Drosophila), protein notum homolog

Entrez Gene IDs

147111 (Human); 77583 (Mouse); 303743 (Rat)

Gene Symbol

NOTUM

UniProt

Q6P988

Additional Notum Products

Product Documents for Human Notum Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Notum Antibody

For research use only

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