Human NPDC-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5489
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human NPDC-1
Gly35-Asp181
Accession # Q9NQX5
Gly35-Asp181
Accession # Q9NQX5
Specificity
Detects human NPDC-1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human NPDC-1 Antibody
Detection of Human NPDC-1 by Western Blot.
Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human NPDC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5489) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for NPDC-1 at approximately 40 kDa and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
NPDC‑1 in Human Brain.
NPDC-1 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Sheep Anti-Human NPDC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5489) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and synaptic vesicles of neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human NPDC-1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)
Western Blot
1 µg/mL
Sample: Human brain tissue
Sample: Human brain tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NPDC-1
NPDC-1 (Neural proliferation differentiation and control protein 1) is a 35 kDa member of the NPDC1/cab1 family of proteins. It is principally expressed in neurons, and serves as an anti-proliferation agent. It may accomplish this, in part, by binding to E2F-1 and blocking its protranscriptional activity. Mature human NPDC-1 is 291 amino acids (aa) in length. It is a type I transmembrane protein that contains an NLS (aa 107‑124), an HLH domain (aa 95‑128), a transmembrane domain (aa 182‑202), and a PEST degradation sequence (aa 269‑302). Phosphorylation promotes ubiquitination at this latter site. There are two splice variants. One shows a deletion of aa 242‑263, while another shows a nine aa substitution for the nine aa in positions 218‑226. Over aa 35‑181, human NPDC-1 shares 68% aa identity with mouse NPDC-1.
Long Name
Neural Proliferation, Differentiation and Control, 1
Alternate Names
CAB1, NPDC1
Gene Symbol
NPDC1
UniProt
Additional NPDC-1 Products
Product Documents for Human NPDC-1 Antibody
Product Specific Notices for Human NPDC-1 Antibody
For research use only
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