Human NPRA/NPR1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB48601
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 377018
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human NPR1/NPRA
Gly33-Glu473
Accession # P16066
Gly33-Glu473
Accession # P16066
Specificity
Detects human NPR1/NPRA in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human NPRA/NPR1 Antibody
NPRA/NPR1 in Human Kidney.
NPRA/NPR1 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human NPRA/NPR1 Monoclonal Antibody (Catalog # MAB48601) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human NPRA/NPR1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NPRA/NPR1
Natriuretic peptide receptor A/guanylate cyclase A (NPR1), also called NPRA or GC-A, is a 120‑140 kDa type I transmembrane glycoprotein that is the primary receptor for natrietic peptides ANP and BNP. Binding of ANP to the extracellular ligand binding domain (aa 54‑415), plus ATP to the intracellular kinase homology domain (aa 528‑805) activates a cytoplasmic guanylate cyclase (aa 840‑1023). NPR1 is expressed most highly in kidney, adrenal and adipose tissue. Human NPR1 extracellular domain shows 86%, 44% and 34% aa identity with mouse NPR1, human NPRB and human NPRC, respectively.
Long Name
Natriuretic Peptide Receptor A
Alternate Names
ANPa, ANPRA, GC-A, GUCY2A, NPR1, NPRA, Pndr
Gene Symbol
NPR1
UniProt
Additional NPRA/NPR1 Products
Product Documents for Human NPRA/NPR1 Antibody
Product Specific Notices for Human NPRA/NPR1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...