Human NPRC/NPR3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10233

R&D Systems, part of Bio-Techne
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MAB10233-100
MAB10233-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 1060839

View all NPRC/NPR3 Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell, HEK293-derived human NPRC/NPR3
Met1-Glu481
Accession # P17342

Specificity

Detects human NPRC/NPR3 in direct ELISA. 

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for Human NPRC/NPR3 Antibody

Detection of NPRC/NPR3 in Human Kidney.

NPRC/NPR3 was detected in immersion fixed paraffin-embedded sections of Human Kidney using Mouse Anti-Human NPRC/NPR3 Monoclonal Antibody (Catalog # MAB10233) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in Parietal cells of Bowman's capsule. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human NPRC/NPR3 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Kidney

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NPRC/NPR3

Atrial Natriuretic Peptide Receptor-3 (NPR3), also known as NPRC or ANPR-C, is one of the three natriuretic peptide receptors (1, 2). Mature human NPR3 is a type I transmembrane glycoprotein that contains a 455 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 37 aa cytoplasmic region. Within the ECD, human NPR3 shares 92% aa sequence identity with mouse and rat NPR3. The natriuretic system is key to the maintenance of vascular tone and cardiovascular homeostasis. It consists of three related natriuretic peptides: atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP). It also consists of three single-membrane-spanning receptors (NPRs) to mediate the biological activity of these peptides: NPR1, NPR2, and NPR3. NPR1 and NPR2 are guanylyl cyclase receptors that regulate cGMP levels, while NPR3 lacks enzymatic activity and may act as a clearance receptor (1, 2). Both ANP and BNP exhibit high binding affinities to NPR1 and NPR3, while CNP binds with high affinity to NPR2 and NPR3 (2). NPR3 is known to be expressed in the heart, lung, adrenal gland, heart, cerebral cortex, cerebellum, liver and adipocytes and in some cancers (1, 2). Osteocrin was found to be a specific ligand to NPR3 (3). NPR3 is necessary for Osteocrin to regulate femoral, tibial, and metatarsal bone elongation (4).

References

  1. Potter, L.R. et al. (2006) Endocr. Rev. 27:47.
  2. Koller, K. J. and D. V. Goeddel (1992) Circulation 86:1081.
  3. Thomas, G. et al. (2003) J. Biol. Chem. 278:50563.
  4. Moffatt, P. et al. (2007) J. Biol. Chem. 282:36454.

Long Name

Natriuretic Peptide Receptor C

Alternate Names

ANP-C, ANPR-C, C5orf23, GUCY2B, lgj, NPR-C, NPR3, stri

Entrez Gene IDs

4883 (Human); 18162 (Mouse); 25339 (Rat)

Gene Symbol

NPR3

UniProt

P17342

Additional NPRC/NPR3 Products

Product Documents for Human NPRC/NPR3 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human NPRC/NPR3 Antibody

For research use only

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