Human NQO-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7567
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human NQO-1
Met1-Lys274
Accession # P15559
Met1-Lys274
Accession # P15559
Specificity
Detects human NQO-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human NQO-2 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human NQO-1 Antibody
Detection of Human NQO-1 by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, human lung tissue, and human kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human NQO-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7567) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NQO-1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human NQO-1 Antibody
Application
Recommended Usage
Western Blot
0.25 µg/mL
Sample: MCF‑7 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, human lung tissue, and human kidney tissue
Sample: MCF‑7 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, human lung tissue, and human kidney tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NQO-1
NQO-1 (NADPH Quinone acceptor Oxidoreductase 1; also DTD and Menadione reductase) is a 31-33 kDa cytoplasmic member of the NAD(P)H dehydrogenase family of enzymes. It is widely expressed, being found in adipocytes, endothelial cells and hepatocytes. NQO-1 serves as a cytoprotective molecule, reducing quinones (atmospheric benzene and esrtrogen compounds) and nitroaromatics without consuming sulfhydryl compounds. It also acts as a backup superoxide scavenger to SOD. NQO-1 reportedly binds and stabilizes key cell protection molecules such as p53, thus acting as a gatekeeper for proteosome-mediated protein turnover. Human NQO-1 is 274 amino acids (aa) in length. It contains an FMN reductase domain (aa 5-212) and two utilized Lys acetylation sites. NQO-1 functions as a noncovalent homodimer. There are three potential at Met22, while two others show a deletion of aa 141-174 and 102-139, respectively. Full length human NQO-1 shares 86% aa sequence identity with mouse NQO-1.
Long Name
NAD(P)H Dehydrogenase, Quinone 1
Alternate Names
Azoreductase, DHQU, DIA4, Diaphorase-4, Dioxin-inducible 1, DTD, Menadione Reductase, NMOR1, NQO1, QR1, Quinone Reductase 1
Entrez Gene IDs
1728 (Human)
Gene Symbol
NQO1
UniProt
Additional NQO-1 Products
Product Documents for Human NQO-1 Antibody
Product Specific Notices for Human NQO-1 Antibody
For research use only
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