Human NRAMP1/SLC11A1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8400
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 910825
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human NRAMP1/SLC11A1
Met1-Gly550
Accession # NP_000569
Met1-Gly550
Accession # NP_000569
Specificity
Detects HEK293 human embryonic kidney cell line transfected with human NRAMP1/SLC11A1 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human NRAMP1/SLC11A1 Antibody
Detection of Human NRAMP1/SLC11A1 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human NRAMP1/SLC11A1. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NRAMP1/SLC11A1 Monoclonal Antibody (Catalog # MAB8400) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for NRAMP1/SLC11A1 at approximately 90-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of NRAMP1/SLC11A1 in HEK293 Human Cell Line Transfected with Human NRAMP1/SLC11A1 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human NRAMP1/SLC11A1 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human NRAMP1/SLC11A1 Monoclonal Antibody (Catalog # MAB8400) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).Applications for Human NRAMP1/SLC11A1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human NRAMP1/SLC11A1 and eGFP
Sample: HEK293 human embryonic kidney cell line transfected with human NRAMP1/SLC11A1 and eGFP
Western Blot
1 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human NRAMP1/SLC11A1
Sample: HEK293 human embryonic kidney cell line transfected with human NRAMP1/SLC11A1
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NRAMP1/SLC11A1
Long Name
Natural Resistance-associated Macrophage Protein 1
Alternate Names
LSH, SLC11A1
Gene Symbol
SLC11A1
UniProt
Additional NRAMP1/SLC11A1 Products
Product Documents for Human NRAMP1/SLC11A1 Antibody
Product Specific Notices for Human NRAMP1/SLC11A1 Antibody
For research use only
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