Human NT-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF-267-NA
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Rat
Applications
Validated:
Immunohistochemistry, Neutralization, Western Blot
Cited:
Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NT-3
Tyr139-Thr257
Accession # P20783
Tyr139-Thr257
Accession # P20783
Specificity
Detects human NT-3 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) NT‑4 and rhBDNF is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human NT-3 Antibody
NT-3 in Rat Brain.
NT-3 was detected in perfusion fixed frozen sections of rat brain (Purkinje cells in the cerebellum) using 10 µg/mL Human NT-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-267-NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Cell Proliferation Induced by NT‑3 and Neutralization by Human NT‑3 Antibody.
Recombinant Human NT-3 (Catalog # 267-N3) stimulates proliferation in BaF-TrKB-BD mouse pro-B cell line transfected with TrkB in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NT-3 (20 ng/mL) is neutralized (green line) by increasing concentrations of Human NT-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-267-NA). The ND50 is typically 0.1-0.4 µg/mL.Applications for Human NT-3 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat brain and immersion fixed parafin embedded human brain (Purkinje cells in the cerebellum)
Sample: Perfusion fixed frozen sections of rat brain and immersion fixed parafin embedded human brain (Purkinje cells in the cerebellum)
Western Blot
0.1 µg/mL
Sample: Recombinant Human NT-3 (Catalog # 267-N3)
Sample: Recombinant Human NT-3 (Catalog # 267-N3)
Neutralization
Measured by its ability to neutralize NT‑3-induced proliferation in BaF‑TrKB-BD mouse pro‑B cell line transfected with TrkB. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 20 ng/mL Recombinant Human NT‑3.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using AF-267-NA in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NT-3
Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer.
The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. NT-3 transcripts have been detected in the cerebellum, hippocampus, placenta, heart, skin, and skeletal muscle. NT-3 primarily activates the TrkC tyrosine kinase receptor. In addition, NT-3 can activate Trk and TrkB kinase receptors in certain cell systems. NT-3 can also bind with low affinity to the low affinity NGF receptor.
References
- Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
- Snider, W.D. (1994) Cell 77:627.
- Barbacid, M. (1994) J. Neurobiol. 25:1386.
Long Name
Neurotrophin 3
Alternate Names
HDNF, NGF2, NT3, NTF3
Entrez Gene IDs
4908 (Human)
Gene Symbol
NTF3
UniProt
Additional NT-3 Products
Product Documents for Human NT-3 Antibody
Product Specific Notices for Human NT-3 Antibody
For research use only
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