Human NT-4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2681

R&D Systems, part of Bio-Techne
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MAB2681
MAB2681-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 36507

View all NT-4 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NT-4
Gly81-Ala210 (predicted)
Accession # P34130

Specificity

Detects human NT-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) BDNF or rhNT-3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human NT-4 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human NT-4 (Catalog # 268-N4)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NT-4

Neurotrophin-4 (NT-4), also known as NT-5, is a member of the NGF family of neuronal and epithelial growth factors. Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds (1‑3). The human NT-4 cDNA encodes a 210 amino acid (aa) precursor that includes a 24 aa signal sequence, a 56 aa propeptide, and a 130 aa mature protein (4, 5). NT-4 is synthesized as a 28 kDa prepropeptide that is proteolytically processed to generate the mature protein. Mature human NT-4 shares 48‑52% aa sequence identity with human beta-NGF, BDNF, and NT-3. It shares 91% and 95% aa sequence identity with mouse and rat NT-4/5, respectively. The mature protein is secreted as a homodimer and can also form heterodimers with BDNF or NT-3 (6). NT-4 binds and induces receptor dimerization and activation of TrkB (4, 7). NT-4 promotes the development and survival of selected peripheral and CNS neurons (8‑10). BDNF, which also activates TrkB, overlaps with many but not all NT-4 functions, a distinction that is likely due to differences in expression patterns (8‑10). NT-4 induced TrkB signaling augments NMDA receptor activity and increases neuronal sensitivity to excitotoxic cell death (11). It also promotes the proliferation of keratinocytes and accelerates hair follicle regression during the follicular cycle (12, 13). NT-4 is secreted by activated T cells and granulocytes at sites of inflammation where it contributes to tissue regeneration (14‑16).

References

  1. Lessmann, V. et al. (2003) Prog. Neurobiol. 69:341.
  2. Tabakman, R. et al. (2004) Prog. Brain Res. 146:387.
  3. Botchkarev, V.A. et al. (2004) Prog. Brain Res. 146:493.
  4. Ip, N.Y. et al. (1992) Proc. Natl. Acad. Sci. 89:3060.
  5. Berkemeier, L.R. et al. (1991) Neuron 7:857.
  6. Radziejewski, C. and R.C. Robinson (1993) Biochemistry 32:13350.
  7. Vesa, J. et al. (2000) J. Biol. Chem. 275:24414.
  8. Davies, A.M. et al. (1993) J. Neurosci. 13:4961.
  9. Stucky, C.L. et al. (1998) J. Neurosci. 18:7040.
  10. Fan, G. et al. (2000) Nat. Neurosci. 3:350.
  11. Choi, S.Y. et al. (2004) J. Neurochem. 88:708.
  12. Botchkarev, V.A. et al. (1999) Lab. Invest. 79:557.
  13. Botchkarev, V.A. et al. (1999) FASEB J. 13:395.
  14. Laurenzi, M.A. et al. (1998) J. Leukoc. Biol. 64:228.
  15. Moalem, G. et al. (2000) J. Autoimmun. 15:331.
  16. Nassenstein, C. et al. (2003) J. Exp. Med. 198:455.

Long Name

Neurotrophin 4

Alternate Names

NT-5, NT4, NTF4, NTF5

Entrez Gene IDs

4909 (Human)

Gene Symbol

NTF4

UniProt

P34130

Additional NT-4 Products

Product Documents for Human NT-4 Antibody

Product Datasheets

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Product Specific Notices for Human NT-4 Antibody

For research use only

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