Human NT-4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB268
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Western Blot
Cited:
ELISA Development, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 36526
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NT-4
Specificity
Detects human NT-4 in direct ELISAs and Western blots. In Western blots, this antibody shows less than 1% cross‑reactivity with recombinant human (rh) NT-3 and rhBDNF.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human NT-4 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human NT-4 (Catalog # 268-N4)
under non-reducing conditions only
Sample: Recombinant Human NT-4 (Catalog # 268-N4)
under non-reducing conditions only
Human NT-4 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NT-4
NT-4 was originally identified as a mammalian homolog of the Xenopus neurotrophin, NT-4. Along with NGF, BDNF, and NT-3, it is a member of the NGF family of neurotrophic factors that are required for the differentiation and survival of specific neuronal subpopulations in both the central and the peripheral nervous systems. NT-4 functions through interactions with the TrkB receptor tyrosine kinase. Because mammalian NT-4 is much more divergent from its amphibian functional counterpart (Xenopus NT-4) than other family members, it is sometimes designated NT-5 or NT-4/5.
Long Name
Neurotrophin 4
Alternate Names
NT-5, NT4, NTF4, NTF5
Entrez Gene IDs
4909 (Human)
Gene Symbol
NTF4
Additional NT-4 Products
Product Documents for Human NT-4 Antibody
Product Specific Notices for Human NT-4 Antibody
For research use only
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