Western blot shows lysates of Raji human Burkitt's lymphoma cell line and THP-1 human acute monocytic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human NTAL Monoclonal Antibody (Catalog # MAB4066) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NTAL at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of NTAL in THP-1 Human Cell Line by Flow Cytometry.

THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human NTAL Monoclonal Antibody (Catalog # MAB4066, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human NTAL Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: THP‑1 human acute monocytic leukemia cell line, fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and THP‑1 human acute monocytic leukemia cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NTAL

Non-T cell activation linker (NTAL), also known as linker for activation of B cells (LAB), is a transmembrane adaptor protein involved in immunoreceptor signaling. NTAL is expressed in lipid raft microdomains of B cells, mast cells, monocytes and NK cells. Rapid tyrosine phosphorylation of NTAL occurs upon BCR aggregation in B cells, Fc epsilonRI aggregation and Kit activation in mast cells, and Fc gammaRI aggregation in monocytes. Phosphorylated NTAL recruits signaling molecules such as Grb2, Gab1, and c-Cbl into receptor-signaling complexes. Defects in the NTAL gene may cause Williams-Beuren syndrome, a rare genetic disorder characterized by mild mental retardation, and abnormalities in the cardiovascular and musculo-skeletal systems.

Long Name

Non-T Cell Activation Linker

Alternate Names

LAB, WBSCR15, WBSCR5

Entrez Gene IDs

7462 (Human); 56743 (Mouse); 317676 (Rat)

Gene Symbol

LAT2

UniProt

Q9GZY6

Additional NTAL Products

Product Documents for Human NTAL Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human NTAL Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human NTAL Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4066

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all NTAL Antibodies »

Product Summary for Human NTAL Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human NTAL Antibody

Detection of Human NTAL by Western Blot.