Western blot shows lysates of Raji human Burkitt's lymphoma cell line and THP-1 human acute monocytic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human NTAL Monoclonal Antibody (Catalog # MAB4066) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NTAL at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of NTAL in THP-1 Human Cell Line by Flow Cytometry.

THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human NTAL Monoclonal Antibody (Catalog # MAB4066, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human NTAL Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: THP‑1 human acute monocytic leukemia cell line, fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and THP‑1 human acute monocytic leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NTAL

Non-T cell activation linker (NTAL), also known as linker for activation of B cells (LAB), is a transmembrane adaptor protein involved in immunoreceptor signaling. NTAL is expressed in lipid raft microdomains of B cells, mast cells, monocytes and NK cells. Rapid tyrosine phosphorylation of NTAL occurs upon BCR aggregation in B cells, Fc epsilonRI aggregation and Kit activation in mast cells, and Fc gammaRI aggregation in monocytes. Phosphorylated NTAL recruits signaling molecules such as Grb2, Gab1, and c-Cbl into receptor-signaling complexes. Defects in the NTAL gene may cause Williams-Beuren syndrome, a rare genetic disorder characterized by mild mental retardation, and abnormalities in the cardiovascular and musculo-skeletal systems.

Long Name

Non-T Cell Activation Linker

Alternate Names

LAB, WBSCR15, WBSCR5

Entrez Gene IDs

7462 (Human); 56743 (Mouse); 317676 (Rat)

Gene Symbol

LAT2

UniProt

Q9GZY6

Additional NTAL Products

Product Documents for Human NTAL Antibody

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for Human NTAL Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human NTAL Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4066

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human NTAL Antibody

Detection of Human NTAL by Western Blot.