Human Oct-4A APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC6344A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 653108
Product Specifications
Immunogen
E. coli-derived recombinant human Oct-4A
Met1-Glu135
Accession # NP_002692
Met1-Glu135
Accession # NP_002692
Specificity
Detects human Oct-4A in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Oct-4A APC-conjugated Antibody
Detection of Oct-4A in BG01V Human Cells by Flow Cytometry.
BG01V human embryonic stem cells were stained with Mouse Anti-Human Oct-4A APC-conjugated Monoclonal Antibody (Catalog # IC6344A, filled histogram) or isotype control antibody (IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.
Detection of Oct-4A in iPSCs by Flow Cytometry.
iPSCs were stained with Mouse Anti-Human Oct-4A Monoclonal Antibody (Catalog # MAB17591, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human Oct-4A APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: BG01V human embryonic stem cells or iPSCs were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: BG01V human embryonic stem cells or iPSCs were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Oct-4A
Oct-3/4, alternately Oct-3 or Oct-4, is POU5F1 (POU domain containing, class 5, transcription factor 1), a 360 amino acid (aa) transcription factor that is expressed in totipotent embryonic stem and germ cells. The human Oct-4, Oct-3/4 or POU5F1 gene can be transcribed into at least three transcripts (Oct-4A, Oct-4B, and Oct-4B1) and generates four protein isoforms by alternative splicing and alternative translation initiation. Oct-4A expression is restricted to embryonic stem (ES) and embryonic carcinoma (EC) cells and is believed to be the transcription factor responsible for the pluripotency properties of embryonic stem (ES) cells. In contrast, Oct-4B/4B1 can be detected in various nonpluripotent cell types and cannot sustain ES cell pluripotency and self-renewal.
Long Name
Octamer-binding Transcription Factor 4A
Alternate Names
Oct4A
Gene Symbol
POU5F1
UniProt
Additional Oct-4A Products
Product Documents for Human Oct-4A APC-conjugated Antibody
Product Specific Notices for Human Oct-4A APC-conjugated Antibody
For research use only
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