Human peripheral blood granulocytes were stained with Rabbit anti-Human OLT-2/TARM1 Monoclonal Antibody (Catalog # MAB10059, filled histogram) or Rabbit IgG control Antibody (MAB1050, open histogram) followed by anti-Rabbit IgG Phycoerythrin-conjugated Secondary Antibody (F0110). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Detection of OLT-2/TARM1 on U937 Cell Line by Flow Cytometry.

U937 human histiocytic lymphoma cell line was stained with Rabbit anti-Human OLT-2/TARM1 Monoclonal Antibody (MAB10059, filled histogram) or Rabbit IgG control Antibody (MAB1050, open histogram) followed by anti-Rabbit IgG Phycoerythrin-conjugated Secondary Antibody (F0110). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Applications for Human OLT-2/TARM1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Peripheral blood granulocytes or U937 human histiocytic lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OLT-2/TARM1

T cell interacting, activating receptor on myeloid cells-1 (TARM-1), also known as OSCAR-like transcript-2 protein (OLT-2) is a type 1 membrane protein expressed by neutrophils, inflammatory monocytes, macrophages, and dendritic cells. Mature mouse OLT-2/TARM-1 contains a 242 aa long extracellular domain with two Ig-like C2 domains, a 21 aa long transmembrane domain and a 9 aa long cytoplasmic domain. The extracellular domain of human OLT-2/TARM-1 shares 48% and 49% amino acid (aa) identity with mouse and rat OLT-2/TARM-1 respectively. OLT-2/TARM-1 associates with the ITAM bearing adaptor FcR gamma, but not with DAP10 or DAP12.OLT-2/TARM1 expression is also up-regulated by bone marrow-derived macrophages and dendritic cells following stimulation with TLR agonists in vitro. OLT-2/TARM-1 receptor stimulation on macrophages and neutrophils co-stimulate the secretion of proinflammatory cytokines induced by TLR ligands, such as LPS. OLT-2/TARM-1 Fc fusion protein inhibits anti-CD3 induced CD4⁺ cell activation, suggesting that OLT-2/TARM-1 ectodomain interacts with an unidentified receptor on T cells to inhibit T cell activation (1).

References

Radjabova, V., et al. (2015) J Immunol. 195:3149.

Long Name

T-cell-interacting Activating Receptor on Myeloid Cells 1

Alternate Names

OLT2, TARM1

Entrez Gene IDs

441864 (Human); 245126 (Mouse); 499065 (Rat)

Gene Symbol

TARM1

UniProt

B6A8C7

Additional OLT-2/TARM1 Products

Product Documents for Human OLT-2/TARM1 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human OLT-2/TARM1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human OLT-2/TARM1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10059

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all OLT-2/TARM1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human OLT-2/TARM1 Antibody

Detection of OLT-2/TARM1 in Peripheral Blood Granulocytes by Flow Cytometry.