Human Osteocalcin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB113271
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 190124
Product Specifications
Immunogen
Osteocalcin/KLH containing peptide
Accession # P02818
Accession # P02818
Specificity
Detects human Osteocalcin in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Osteocalcin Antibody
Human Osteocalcin ELISA Standard Curve.
Recombinant Human Osteocalcin protein was serially diluted 2-fold and captured by Mouse Anti-Human Osteocalcin Monoclonal Antibody (MAB11327) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Osteocalcin Monoclonal Antibody (Catalog # MAB113271) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human Osteocalcin Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Osteocalcin Monoclonal Antibody (Catalog # MAB11327).This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Osteocalcin DuoSet ELISA Kit (Catalog # DY1419-05) for convenient development of a sandwich ELISA.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Osteocalcin
References
- Lian, J.B. et al. (1999) Vitamin. Horm. 55:443.
Long Name
Bone gamma-Carboxyglutamate [gla] Protein
Alternate Names
BGLAP, BGP, OCN
Gene Symbol
BGLAP
UniProt
Additional Osteocalcin Products
Product Documents for Human Osteocalcin Antibody
Product Specific Notices for Human Osteocalcin Antibody
For research use only
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