Human Osterix/Sp7 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7547
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 764704
Product Specifications
Immunogen
E. coli-derived recombinant human Osterix/Sp7
Met19-Leu288
Accession # Q8TDD2
Met19-Leu288
Accession # Q8TDD2
Specificity
Detects human Osterix/Sp7 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Osterix/Sp7 Antibody
Detection of Human Osterix/Sp7 by Western Blot.
Western blot shows lysates of Saos-2 human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Osterix/Sp7 Monoclonal Antibody (Catalog # MAB7547) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Osterix/Sp7 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Osterix/Sp7 in Saos‑2 Human Cell Line.
Osterix/Sp7 was detected in immersion fixed Saos-2 human osteosarcoma cell line using Mouse Anti-Human Osterix/Sp7 Monoclonal Antibody (Catalog # MAB7547) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Osterix/Sp7 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed Saos‑2 human osteosarcoma cell line
Sample: Immersion fixed Saos‑2 human osteosarcoma cell line
Western Blot
1 µg/mL
Sample: Saos‑2 human osteosarcoma cell line
Sample: Saos‑2 human osteosarcoma cell line
Reviewed Applications
Read 1 review rated 4 using MAB7547 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Osterix/Sp7
Long Name
Sp7 Transcription Factor
Alternate Names
OSX, Sp7
Gene Symbol
SP7
UniProt
Additional Osterix/Sp7 Products
Product Documents for Human Osterix/Sp7 Antibody
Product Specific Notices for Human Osterix/Sp7 Antibody
For research use only
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