Human P-Selectin/CD62P Antibody
R&D Systems, part of Bio-Techne | Catalog # BBA30
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Adhesion Blockade, Immunohistochemistry
Cited:
Immunohistochemistry, Immunohistochemistry-Frozen, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 9E1
Product Specifications
Immunogen
Recombinant human P-Selectin
Extracellular domain
Extracellular domain
Specificity
This antibody binds to CHO cells transfected with human P-Selectin but not to CHO cells transfected with either human E-Selectin or human L-Selectin.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human P-Selectin/CD62P Antibody
P-Selectin/CD62P in Human Breast Cancer Tissue.
P-Selectin/CD62P was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Human P-Selectin/CD62P Monoclonal Antibody (Catalog # BBA30) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human P-Selectin/CD62P Antibody
Application
Recommended Usage
Adhesion Blockade
The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human P-Selectin/CD62P (Catalog # ADP3, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 1 µg/mL of the antibody.
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Reviewed Applications
Read 1 review rated 4 using BBA30 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: P-Selectin/CD62P
Alternate Names
CD62P, GMP140, GRMP, PADGEM, PSEL, SELP
Gene Symbol
SELP
Additional P-Selectin/CD62P Products
Product Documents for Human P-Selectin/CD62P Antibody
Product Specific Notices for Human P-Selectin/CD62P Antibody
For research use only
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