Human p15INK4b/CDKN2B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6798
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 651308
Product Specifications
Immunogen
E. coli-derived recombinant human p15INK4b/CDKN2B
Arg2-Asp138
Accession # P42772
Arg2-Asp138
Accession # P42772
Specificity
Detects human p15INK4b/CDKN2B in direct ELISAs and Western blots.
In Western blots, approximately 50%
cross-reactivity with recombinant human (rh) p16INK4a and no cross-reactivity
with rhp18INK4c or rhp19INK4d is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human p15INK4b/CDKN2B Antibody
Detection of Human p15INK4b/CDKN2B by Western Blot.
Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human p15INK4b/CDKN2B Monoclonal Antibody (Catalog # MAB6798) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p15INK4b/CDKN2B at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
p15INK4b/CDKN2B in HeLa Human Cell Line.
p15INK4b/CDKN2B was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human p15INK4b/CDKN2B Monoclonal Antibody (Catalog # MAB6798) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human p15INK4b/CDKN2B Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line
Sample: 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p15INK4b/CDKN2B
Long Name
p15 Cyclin Dependent Kinase 4 Inhibitor 2B
Alternate Names
CDKN2B, MTS2
Gene Symbol
CDKN2B
UniProt
Additional p15INK4b/CDKN2B Products
Product Documents for Human p15INK4b/CDKN2B Antibody
Product Specific Notices for Human p15INK4b/CDKN2B Antibody
For research use only
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