Human p21/CIP1/CDKN1A Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF1047
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human p21/CIP1/CDKN1A
Ser2-Pro164
Accession # P38936
Ser2-Pro164
Accession # P38936
Specificity
Detects human p21/CIP1/CDKN1A in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human p21/CIP1/CDKN1A Biotinylated Antibody
p21/CIP1/CDKN1A in Human Breast.
p21/CIP1/CDKN1A was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human p21/CIP1/CDKN1A Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1047) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in ductal epithelial cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human p21/CIP1/CDKN1A Biotinylated Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast
Sample: Immersion fixed paraffin-embedded sections of human breast
Western Blot
0.1 µg/mL
Sample: Recombinant Human p21/CIP1/CDKN1A
Sample: Recombinant Human p21/CIP1/CDKN1A
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p21/CIP1/CDKN1A
Long Name
p21 Cyclin Dependent Kinase 4 Inhibitor 1A
Alternate Names
CDKN1A, CIP1
Gene Symbol
CDKN1A
UniProt
Additional p21/CIP1/CDKN1A Products
Product Documents for Human p21/CIP1/CDKN1A Biotinylated Antibody
Product Specific Notices for Human p21/CIP1/CDKN1A Biotinylated Antibody
For research use only
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