Human P2X7/P2RX7 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB97902
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 1058610
Product Summary for Human P2X7/P2RX7 Antibody
Immunogen
Human P2X7/P2RX7 synthetic peptide
Accession # Q99572
Accession # Q99572
Specificity
Detects human P2X7/P2RX7 in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human P2X7/P2RX7 Antibody
Detection of P2X7/P2RX7 in Lung Cancer.
P2X7/P2RX7 was detected in immersion fixed paraffin-embedded sections of Lung Cancer using Mouse Anti-Human P2X7/P2RX7 Monoclonal Antibody (Catalog # MAB97902) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human P2X7/P2RX7 Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Lung Cancer
Sample: Immersion fixed paraffin-embedded sections of Lung Cancer
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: P2X7/P2RX7
Human P2X purinoceptor 7 (aka P2X7) is a 595 aminoacids (aa) protein encoded by the P2RX7 gene. P2X7 receptors belong to the family of ATP-gated ion channels, and their activity can be found in cells of hemopoietic lineage including macrophages, microglia, and certain lymphocytes. They mediate the influx of Ca2+ and Na+ and the release of pro-inflammatory cytokines. At least 8 different human P2X7 isoforms formed by alternative splicing have been reported, ranging from 128 aa to 595 aa. The P2X7 subunits can form homomeric receptors only with a typical P2X receptor structure.
Long Name
P2X Purinergic Receptor 7
Alternate Names
P2RX7
Gene Symbol
P2RX7
UniProt
Additional P2X7/P2RX7 Products
Product Documents for Human P2X7/P2RX7 Antibody
Product Specific Notices for Human P2X7/P2RX7 Antibody
For research use only
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