HEK293 human embryonic kidney cell line transfected with either (A) human P2Y11/P2RY11 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human P2Y11/P2RY11 Monoclonal Antibody (Catalog # MAB9305) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Applications for Human P2Y11/P2RY11 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human P2Y11/P2RY11 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: P2Y11/P2RY11

P2RY11 belongs to the family of G protein-coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. P2RY11 is coupled to the stimulation of thephosphoinositide and adenylyl cyclase pathways and behaves as a selective purinoceptor. Naturally occuring read-through transcripts,resulting from intergenic splicing between P2RY11 and an immediately upstream gene (PPAN, encoding peter pan homolog), have been found. Variants in the P2RY11 gene have been associated with narcolepsy.

Long Name

P2Y Purinergic Receptor 11

Alternate Names

P2RY11

Entrez Gene IDs

5032 (Human); 235036 (Mouse); 298699 (Rat)

Gene Symbol

P2RY11

UniProt

Q96G91

Additional P2Y11/P2RY11 Products

Product Documents for Human P2Y11/P2RY11 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human P2Y11/P2RY11 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human P2Y11/P2RY11 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9305

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all P2Y11/P2RY11 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human P2Y11/P2RY11 Antibody

Detection of P2Y11/P2RY11 in HEK293 Human Cell Line Transfected with Human P2Y11/P2RY11 and eGFP by Flow Cytometry.