Human PAR1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3855
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PAR1
Arg27-Thr102, Ser375-Thr425
Accession # P25116
Arg27-Thr102, Ser375-Thr425
Accession # P25116
Specificity
Detects human PAR1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human PAR1 Antibody
Detection of PAR1 in HT‑29 Human Cell Line by Flow Cytometry.
HT-29 human colon adenocarcinoma cell line was stained with Human PAR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3855, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
PAR1 in HT‑29 Human Cell Line.
PAR1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Goat Anti-Human PAR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3855) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human PAR1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: HT-29 human colon adenocarcinoma cell line
Sample: HT-29 human colon adenocarcinoma cell line
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HT-29 human colon adenocarcinoma cell line
Sample: Immersion fixed HT-29 human colon adenocarcinoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human PAR1
Sample: Recombinant Human PAR1
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PAR1
Human Proteinase-Activated Receptor 1 (hPAR1), also known as thrombin receptor, is a 65-70 kDa, 399 amino acid long member of the seven-transmembrane superfamily of cell-surface G protein-coupled receptors. PAR1 is activated by thrombin cleavage of its N-terminal propeptide in the extracellular domain. Human PAR1 is widely expressed in many cell types including endothelial cells, and it has been implicated in a variety of inflammatory responses. Over the regions used as immunogen, human and mouse PAR1 proteins are 58% identical in the region spanning the propeptide and extracellular domains, and 84% identical in the cytoplasmic tail.
Long Name
Protease-Activated Receptor 1
Alternate Names
Cf2r, F2R, HTR, ThrR, TRGPC
Gene Symbol
F2R
UniProt
Additional PAR1 Products
Product Documents for Human PAR1 Antibody
Product Specific Notices for Human PAR1 Antibody
For research use only
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