Human PARD3/Par3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8030
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry
Cited:
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 833517
Product Specifications
Immunogen
E. coli-derived recombinant human PARD3/Par3
Tyr451-Glu555
Accession # Q8TEW0
Tyr451-Glu555
Accession # Q8TEW0
Specificity
Detects human PARD3/Par3 in ELISAs.
In direct ELISAs, 100% cross-reactivity with recombinant human PARD3b/Par3b is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human PARD3/Par3 Antibody
PARD3/Par3 in HEK293 Human Cell Line.
PARD3/Par3 was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human PARD3/Par3 Monoclonal Antibody (Catalog # MAB8030) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human PARD3/Par3 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line
Sample: Immersion fixed HEK293 human embryonic kidney cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PARD3/Par3
Long Name
Par-3 Partitioning Defective 3 Homolog
Alternate Names
ASIP, Baz, Bazooka, PAR3, PAR3A, SE2-5L16, SE2-5LT1, SE2-5T2
Gene Symbol
PARD3
UniProt
Additional PARD3/Par3 Products
Product Documents for Human PARD3/Par3 Antibody
Product Specific Notices for Human PARD3/Par3 Antibody
For research use only
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