Human Patched 2/PTCH2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4078
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 403438
Product Specifications
Immunogen
E. coli-derived recombinant human Patched 2/PTCH2
Glu79-Glu392
Accession # Q9Y6C5
Glu79-Glu392
Accession # Q9Y6C5
Specificity
Detects human Patched 2/PTCH2 in direct ELISA and Western Blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Patched 2/PTCH2 Antibody
Detection of Human Patched 2/PTCH2 by Western Blot.
Western blot shows lysates of OVCAR-3 human ovarian carcinoma cell line, WS-1 human fetal skin fibroblast cell line, and Bowes human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Patched 2/PTCH2 Monoclonal Antibody (Catalog # MAB4078) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Patched 2/PTCH2 at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Patched 2/PTCH2 in Human Cervical Cancer Tissue.
Patched 2/PTCH2 was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Mouse Anti-Human Patched 2/PTCH2 Monoclonal Antibody (Catalog # MAB4078) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Patched 2/PTCH2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human cervical cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human cervical cancer tissue
Western Blot
1 µg/mL
Sample: OVCAR‑3 human ovarian carcinoma cell line, WS‑1 human fetal skin fibroblast cell line, and Bowes human melanoma cell line
Sample: OVCAR‑3 human ovarian carcinoma cell line, WS‑1 human fetal skin fibroblast cell line, and Bowes human melanoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Patched 2/PTCH2
Alternate Names
ptc2, PTCH2
Gene Symbol
PTCH2
UniProt
Additional Patched 2/PTCH2 Products
Product Documents for Human Patched 2/PTCH2 Antibody
Product Specific Notices for Human Patched 2/PTCH2 Antibody
For research use only
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