Human Patched 2/PTCH2 (First Extracellular Loop) Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4078
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Patched 2/PTCH2 first extracellular loop
Glu79-Glu392
Accession # Q9Y6C5
Glu79-Glu392
Accession # Q9Y6C5
Specificity
Detects human Patched 2/PTCH2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse Patched is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Patched 2/PTCH2 (First Extracellular Loop) Antibody
Detection of Patched 2 in WS-1 Human Cell Line by Flow Cytometry.
WS-1 human fetal skin fibroblast cell line was stained with Human Patched 2 (First Extracellular Loop) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4078, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Patched 2 in Human Basal Cell Carcinoma.
Patched 2 was detected in immersion fixed paraffin-embedded sections of human basal cell carcinoma using 3 µg/mL Goat Anti-Human Patched 2 (First Extracellular Loop) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4078) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Patched 2/PTCH2 (First Extracellular Loop) Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human basal cell carcinoma subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human basal cell carcinoma subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: WS-1 human fetal skin fibroblast cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: WS-1 human fetal skin fibroblast cell line fixed with paraformaldehyde and permeabilized with saponin
Western Blot
0.1 µg/mL
Sample: Recombinant Human Patched 2 (First Extracellular Loop)
Sample: Recombinant Human Patched 2 (First Extracellular Loop)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Patched 2/PTCH2
Patched 2 (Ptch2) is a 180-200 kDa 12 transmembrane member of the Patched family. Human PTCH2 is 1203 amino acids (aa) in length. It has two terminal cytoplasmic domains, one between aa 1-57, and a second between aa 1115-1203. Together with Smoothened (SMO), Ptch2 forms a membrane complex that serves as a receptor for Sonic Hedgehog (SHH). Human Ptch2 likely has two isoforms. One shows an eight aa extension of the C-terminus, while a second shows a four aa substitution for the C-terminal 61 amino acids (aa 1143-1203). Over the first extracellular loop human Patched 2 shares 95% and 96% aa sequence identity with mouse and canine Patched 2, respectively.
Alternate Names
ptc2, PTCH2
Gene Symbol
PTCH2
UniProt
Additional Patched 2/PTCH2 Products
Product Documents for Human Patched 2/PTCH2 (First Extracellular Loop) Antibody
Product Specific Notices for Human Patched 2/PTCH2 (First Extracellular Loop) Antibody
For research use only
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