Human PBEF/Visfatin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4335

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB4335-100
MAB4335-SP
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Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 882115R

View all PBEF/Visfatin Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant Human PBEF/Visfatin
Pro27-His491
Accession # P43490

Specificity

Detects human PBEF/Visfatin in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human PBEF/Visfatin Antibody

Human PBEF/Visfatin Antibody in ELISA Standard Curve.

Human PBEF/Visfatin ELISA Standard Curve.

Recombinant Human PBEF/Visfatin protein was serially diluted 2-fold and captured by Mouse Anti-Human PBEF/Visfatin Monoclonal Antibody (Catalog # MAB4335) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human PBEF/Visfatin Monoclonal Antibody (Catalog # MAB43351) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human PBEF/Visfatin Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human PBEF/Visfatin Monoclonal Antibody (Catalog # MAB43351).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human PBEF/Visfatin DuoSet ELISA Kit (Catalog # DY4335-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PBEF/Visfatin

PBEF (pre-B cell colony-enhancing factor), also known as visfatin and nicotinamide phosphoribosyltransferase, is an approximately 52 kDa member of the NAPRTase family of molecules. It functions both intracellularly and extracellularly, where it participates in NAD synthesis and insulin receptor activation, respectively. Human PBEF is 491 amino acids in length and contains no signal sequence. There is at least one alternative splice form that shows a 5 aa substitution for the C-terminal 128 amino acids (aa 364-491). Over aa 27-491, human PBEF shares 96%, 97%, and 96% aa identity with mouse, porcine, and canine PBEF, respectively.

Long Name

Pre-B-Cell Colony-Enhancing Factor 1

Alternate Names

NAmPRTase, NAMPT, NMPRTase, PBEF1, Visfatin

Entrez Gene IDs

10135 (Human); 59027 (Mouse); 297508 (Rat)

Gene Symbol

NAMPT

UniProt

P43490

Additional PBEF/Visfatin Products

Product Documents for Human PBEF/Visfatin Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PBEF/Visfatin Antibody

For research use only

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