Human PDGF-AA Antibody
R&D Systems, part of Bio-Techne | Catalog # AF-221-NA
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Neutralization, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PDGF-AA
Ser87-Thr211
Accession # P04085
Ser87-Thr211
Accession # P04085
Specificity
Detects human PDGF-AA and PDGF‑AB in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human PDGF-BB and recombinant rat PDGF-BB is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human PDGF-AA Antibody
Cell Proliferation Induced by PDGF-AA and Neutralization by Human PDGF-AA Antibody.
Recombinant Human PDGF-AA (Catalog # 221-AA) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-AA (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF-AA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-221-NA). The ND50 is typically 0.1-0.3 µg/mL.
PDGF‑AA in Human Breast and Breast Cancer Tissue.
PDGF-AA was detected in immersion fixed paraffin-embedded sections of human breast and breast cancer tissue using Goat Anti-Human PDGF-AA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-221-NA) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human PDGF-AA Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Western Blot
0.1 µg/mL
Sample: Recombinant Human PDGF-AA (Catalog # 221-AA)
Sample: Recombinant Human PDGF-AA (Catalog # 221-AA)
Neutralization
Measured by its ability to neutralize PDGF‑AA-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The Neutralization Dose (ND50) is typically 0.1-0.3 µg/mL in the presence of 25 ng/mL Recombinant Human PDGF‑AA.
Reviewed Applications
Read 1 review rated 3 using AF-221-NA in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDGF-AA
alpha-granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.
Long Name
Platelet-derived Growth Factor AA
Alternate Names
PDGFAA
Gene Symbol
PDGFA
UniProt
Additional PDGF-AA Products
Product Documents for Human PDGF-AA Antibody
Product Specific Notices for Human PDGF-AA Antibody
For research use only
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