Human PDGF-D Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1159

R&D Systems, part of Bio-Techne
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BAF1159
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AF1159
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all PDGF-D Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PDGF-D

Specificity

Detects human PDGF-D in Western blots. In this format, less than 1% cross-reactivity with recombinant human (rh) PDGF-AA, rhPDGF-AB and rhPDGF-BB is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human PDGF-D Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human PDGF-D

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-D

The platelet-derived growth factor (PDGF) family consists of four disulfide-linked homodimers and one heterodimer (PDGF-AB). These proteins regulate diverse cellular functions through interactions with PDGF R alpha and R beta (1, 2). Mature PDGF-DD associates with PDGF R beta and triggers signaling through PDGF R beta homodimers and PDGF R alpha/ beta heterodimers (3 - 5). The human PDGF-DD cDNA encodes a 370 amino acid (aa) precursor that includes a 23 aa signal sequence, one CUB domain, and one PDGF/VEGF domain (3, 4). The PDGF/VEGF domain shares 27 - 35% aa sequence identity with the corresponding regions of other PDGF family members. Human PDGF-DD shares 87% aa sequence identity with mouse and rat PDGF-DD. PDGF-DD is secreted as a100 kDa latent homodimer which is activated by proteolysis to release a 35 kDa bioactive protein containing the PDGF/VEGF homology domain (3 ,4 ,6 ,7). A splice variant of PDGF-DD has a 6 aa deletion near the N-terminus. A 72 aa deletion within the PDGF/VEGF domain generates an inactive protein in mouse but has not been detected in human (8). PDGF-DD is widely expressed in embryonic and adult tissues (3, 9, 10), and PDGF R beta is expressed in a generally complementary pattern (9, 11, 12). PDGF-DD functions as a growth factor for renal artery smooth muscle cells and lens epithelial cells, and as a macrophage chemoattractant (5, 9 - 11). PDGF-DD is overexpressed in and contributes to several disease states, including renal and hepatic fibrosis, mesangial proliferative glomerulopathy, pulmonary lymphoid infiltration, and many cancers (6, 11 - 15). PDGF-DD functions in both paracrine and autocrine manners (6, 7, 14).

References

  1. Reigstad, L.J. et al. (2005) FEBS J. 272:5723.
  2. Fredriksson, L. et al. (2004) Cytokine Growth Factor Rev. 15:197.
  3. LaRochelle, W.J. et al. (2001) Nat. Cell Biol. 3:517.
  4. Bergsten, E. et al. (2001) Nat. Cell Biol. 3:512.
  5. Uutela, M. et al. (2004) Blood 104:3198.
  6. Ustach, C.V. and H-R.C. Kim (2005) Mol. Cell. Biol. 25:6279. 
  7. Ustach, C.V. et al. (2004) Canc. Res. 64:1722.
  8. Zhuo, Y. et al. (2003) Biochem. Biophys. Res. Commun. 308:126.
  9. Changsirikulchai, S. et al. (2002) Kid. Int. 62:2043.
  10. Ray, S. et al. (2005) J. Biol. Chem. 280:8494.
  11. Hudkins, K.L. et al. (2004) J. Am. Soc. Nephrol. 15:286.
  12. Lokker, N.A. et al. (2002) Canc. Res. 62:3729.
  13. Taneda, S. et al. (2003) J. Am. Soc. Nephrol. 14:2544.
  14. LaRochelle, W.J. et al. (2002) Canc. Res. 62:2468.
  15. Xu, L. et al. (2005) Canc. Res. 65:5711.

Long Name

Platelet-derived Growth Factor D

Alternate Names

PDGFD

Entrez Gene IDs

80310 (Human); 71785 (Mouse); 66018 (Rat)

Gene Symbol

PDGFD

Additional PDGF-D Products

Product Documents for Human PDGF-D Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human PDGF-D Biotinylated Antibody

For research use only

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