Human PDGF R alpha Alexa Fluor® 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1264G
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # PRa292
Product Specifications
Immunogen
Human osteosarcoma cell membrane extracts
Specificity
Detects human PDGF R alpha. Recognizes the PDGF receptor alpha-subunit; it does not recognize the PDGF receptor beta-subunit. Binds to the PDGF receptor alpha-subunit of primate species (human, monkey, baboon) and dog. Does not recognize rat or mouse receptors and its ability to bind to receptors from other species has not been tested.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images
Detection of PDGF R alpha in U‑118‑MG Human Cell Line by Flow Cytometry.
U-118-MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human PDGF R alpha Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1264G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of PDGF R alpha in MG-63 Human Cell Line by Flow Cytometry.
MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human PDGF R alpha Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1264G, filled histogram) or isotype control antibody (IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: U‑118‑MG human glioblastoma/astrocytoma cell line and MG-63 human osteosarcoma cell line
Sample: U‑118‑MG human glioblastoma/astrocytoma cell line and MG-63 human osteosarcoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PDGF R alpha
PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF‑BB and AB, but not PDGF-AA. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and heterodimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Only PDGF R alpha is expressed in oligodendrocyte progenitor cells, mesothelial cell and liver endothelial cells. Soluble PDGF-R alpha has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF R alpha binds PDGF with high affinity and is a potent PDGF antagonist (1).
References
- Heldin, C.H. and L. Claesson-Welsh (1994) Guidebook to Cytokines and Their Receptors, Nicola, N.A. (ed) Oxford University Press, New York, NY p. 202.
Long Name
Platelet-derived Growth Factor Receptor alpha
Alternate Names
CD140a, PDGFR alpha, PDGFRA
Gene Symbol
PDGFRA
Additional PDGF R alpha Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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