Human Pepsinogen A5/PGA5 Antibody

Name: Human Pepsinogen A5/PGA5 Antibody
Brand: R&D Systems
SKU: MAB8457
Rating: 5 (1 reviews)

R&D Systems, part of Bio-Techne | Catalog # MAB8457

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Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 974731

View all Pepsinogen A5/PGA5 Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell, HEK293-derived human Pepsinogen A5/PGA5 protein
Ile16-Ala388
Accession # P0DJD9

Specificity

Detects human Pepsinogen A5/PGA5 protein in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Pepsinogen A5/PGA5 Antibody

Detection of Human Pepsinogen A5/PGA5 by Western Blot.

Western blot shows lysates of human stomach cancer tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Pepsinogen A5/PGA5 Monoclonal Antibody (Catalog # MAB8457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Pepsinogen A5/PGA5 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Pepsinogen A5/PGA5 in Human Stomach.

Pepsinogen A5/PGA5 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human Pepsinogen A5/PGA5 Monoclonal Antibody (Catalog # MAB8457) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intestinal glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human Pepsinogen A5/PGA5 by Simple Western^TM.

Simple Western lane view shows lysates of human stomach tissue and human stomach cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for Pepsinogen A5/PGA5 at approximately 48 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Pepsinogen A5/PGA5 Monoclonal Antibody (Catalog # MAB8457) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Human Pepsinogen A5/PGA5 Antibody

Application

Recommended Usage

Immunohistochemistry

0.5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach

Simple Western

20 µg/mL
Sample: Human stomach tissue and Human stomach cancer tissue

Western Blot

2 µg/mL
Sample: Human stomach cancer tissue

Reviewed Applications

Read 1 review rated 5 using MAB8457 in the following applications:

Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pepsinogen A5/PGA5

Pepsins are aspartic proteases that are synthesized in the gastric mucosa and secreted into the stomach. They are released as zymogens called pepsinogens which are then converted to active pepsins by the acidic pH of gastric juices (1-3). PGA3, PGA4, and PGA5 are human Pepsinogen A isozymogens that differ in sequence by 24 amino acid (aa) residues (4, 5). This recombinant human Pepsinogen A corresponds to PGA5. Human Pepsinogen A isozymogens share approximately 56% aa sequence identity with mouse and rat Pepsinogen A isozymogens. Pepsins have optimal activity under conditions of acidic pH and are inhibited by pepstatin (6, 7). Pepsin A has broad substrate specificity, but preferentially cleaves peptide bonds involving aromatic and aliphatic amino acids.

References

Athauda, S.B. et al. (1989) J. Biochem. 106:920.
Kageyama, T. et al. (1989) J. Biochem. 105:15.
Kageyama, T. (2002) Cell. Mol. Life Sci. 59:288.
Zwiers, A. et al. (1994) Clin. Nephrol. 41:153.
Nakai, H. et al. (1986) Cytogenet. Cell Genet. 43:215.
Marciniszyn, J., Jr. et al. (1976) J. Biol. Chem. 251:7088.
Kageyama, T. and K. Takahashi (1980) J. Biochem. 88:571.

Alternate Names

PGA5

Entrez Gene IDs

5222 (Human)

Gene Symbol