Human PER1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4565
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PER1
Ser159-Thr365
Accession # O15534
Ser159-Thr365
Accession # O15534
Specificity
Detects human PER1 in direct ELISAs.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human PER1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex and hypothalamus)
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex and hypothalamus)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PER1
PER1 (Period circadian protein 1; also known as RIGUI) is a 188 kDa member of the PER family of molecules. It apparently influences circadian rhythms by interacting with, and stabilizing, other circadian regulatory proteins. PER1 undergoes extensive phosphorylation that may regulate its intracellular localization. Human PER1 is 1290 amino acids (aa) in length. It contains a bHLH region (aa 122-173), an extended PAS domain (aa 183-463) plus an NLS and NES. Multiple splice forms exist. Two show deletions (aa 741-820 and aa 209-217), while two show aa substitutions: 9 aa for the C-terminal 1005 aa, and 29 aa for the C-terminal 747 amino acids. Over aa 159-365, human PER1 is 96% identical to mouse, rat, and dog PER1.
Long Name
Period Circadian Protein 1
Alternate Names
PERIOD1, RIGUI
Gene Symbol
PER1
UniProt
Additional PER1 Products
Product Documents for Human PER1 Antibody
Product Specific Notices for Human PER1 Antibody
For research use only
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