Human Perforin Alexa Fluor® 750-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC103853S
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Pro22-Trp555
Accession # P14222
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Perforin Alexa Fluor® 750-conjugated Antibody
Detection of Perforin in NK cells by Flow Cytometry.
NK cells expanded and activated (CCM032) for 14 days were harvested and stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (FAB2408A) and Mouse Anti-Hunan CD3 Alexa Fluor® 405, and either (A) Mouse Anti-Human Perforin Alexa Fluor® 750-conjugated Monoclonal Antibody (Catalog # IC103853S) or (B) Mouse IgG2A Alexa Fluor® 750-conjugated Isotype Control (IC003S). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.Detection of Perforin in PBMC lymphocytes by Flow Cytometry.
PBMC lymphocytes were stained with Rabbit Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB24086P) and either (A) Mouse Anti-Human Perforin Alexa Fluor® 750-conjugated Monoclonal Antibody (Catalog # IC103853S) or (B) Mouse IgG2A Alexa Fluor® 750-conjugated Isotype Control (Catalog # IC003S). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.Applications for Human Perforin Alexa Fluor® 750-conjugated Antibody
Intracellular Staining by Flow Cytometry
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Perforin
Long Name
Alternate Names
Gene Symbol
UniProt
Additional Perforin Products
Product Specific Notices for Human Perforin Alexa Fluor® 750-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only