Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 1031751

View all Perforin Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line CHO-derived human Perforin
Pro22-Trp555
Accession # P14222

Specificity

Detects human Perforin in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Perforin Alexa Fluor® 750-conjugated Antibody

Detection of Perforin in NK cells by Flow Cytometry.

NK cells expanded and activated (CCM032) for 14 days were harvested and stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (FAB2408A) and Mouse Anti-Hunan CD3 Alexa Fluor® 405, and either (A) Mouse Anti-Human Perforin Alexa Fluor® 750-conjugated Monoclonal Antibody (Catalog # IC103853S) or (B) Mouse IgG2A Alexa Fluor® 750-conjugated Isotype Control (IC003S). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.

Detection of Perforin in PBMC lymphocytes by Flow Cytometry.

PBMC lymphocytes were stained with Rabbit Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB24086P) and either (A) Mouse Anti-Human Perforin Alexa Fluor® 750-conjugated Monoclonal Antibody (Catalog # IC103853S) or (B) Mouse IgG2A Alexa Fluor® 750-conjugated Isotype Control (Catalog # IC003S). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human Perforin Alexa Fluor® 750-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

Titration recommended for optimal concentration with starting range of 0.1-1 µg/1 million cells. Samples used for this experiment were NK cells expanded and activated (Catalog # CCM032) for 14 days were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012) and PBMC lymphocytes fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit  (Catalog # FC012).

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Perforin

Perforin is a pore forming, cytolytic protein that is a component of cytolytic T-cell and NK cell granules.  Perforin activates clathrin- and dynamin-dependent endocytosis, which removes perforin and granzymes from the plasm membrane to early endosomes, preserving outer membrane integrity.  Perforin facilitates granzyme uptake and avoids the proinflammatory necrotic death of a membrane-damaged cell.  Defects in the Perforin gene results in Familial hemophagocytic lymphohistiocytosis (FHL).  Perforin-based effector systems are involved not only in the lysis of abnormal cells but also in the down-regulation of cellular immune activation.

Long Name

Perforin 1 (Pore Forming Protein)

Alternate Names

Cytolysin, FLH2, HPLH2, P1, PFP, PRF1

Entrez Gene IDs

5551 (Human); 18646 (Mouse); 50669 (Rat)

Gene Symbol

PRF1

UniProt

P14222

Additional Perforin Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Perforin Alexa Fluor® 750-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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