Human Perilipin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6615
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Perilipin
Thr8-Ala145
Accession # O60240
Thr8-Ala145
Accession # O60240
Specificity
Detects human Perilipin in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Perilipin Antibody
Detection of Human Perilipin by Western Blot.
Western blot shows lysates of human heart (adipose) tissue. PVDF Membrane was probed with 0.25 µg/mL of Human Perilipin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6615) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Perilipin at approximately 55-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Perilipin Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human heart (adipose) tissue
Sample: Human heart (adipose) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Perilipin
Perilipin (Perilipin-1/A; also PLIN1 and Lipid droplet-associated protein) is a 62 kDa constitutively phosphorylated protein that belongs to the PAT (Perilipin/Adipophilin/TIP47) family of molecules. Found primarily in mature adipocytes, perilipin-1 interacts with lipid-coat proteins at the edge of the fat droplet and blocks lipase activity. Upon phosphorylation, perilipin-1 becomes a 65-67 kDa molecule that likely promotes the dispersion of docking molecules and provides a scaffold for lipase at the lipid droplet surface. Human perilipin-1 is 522 amino acids (aa) in length. It contains a lipid droplet targeting region (aa 233-364) that contains a polyGlu segment (aa 307-316). Phosphorylation at the N-terminus is necessary for lipase interaction with lipid. One 50-54 kDa human splice variant (perilipin-B) is reported that shows an 85 aa substitution for aa 404-522. This apparently interacts with cell membrane triglycerides. Over aa 8-145, human perilipin-1/A shares 97% aa identity with mouse perilipin-1.
Alternate Names
PERI, PLIN, PLIN1
Gene Symbol
PLIN1
UniProt
Additional Perilipin Products
Product Documents for Human Perilipin Antibody
Product Specific Notices for Human Perilipin Antibody
For research use only
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