Human Periostin/OSF-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB35484
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG2A Clone # 613643R
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Periostin/OSF-2
Met1-Gln836
Accession # Q15063
Met1-Gln836
Accession # Q15063
Specificity
Detects human Periostin/OSF-2 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Periostin/OSF-2 Antibody
Human Periostin/OSF‑2 ELISA Standard Curve.
Recombinant Human Periostin/OSF-2 protein was serially diluted 2-fold and captured by Mouse Anti-Human Periostin/OSF-2 Monoclonal Antibody (Catalog # MAB35483) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Periostin/OSF-2 Monoclonal Antibody (Catalog # MAB35484) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human Periostin/OSF-2 Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Periostin/OSF‑2 Monoclonal Antibody (Catalog # MAB35483).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Periostin/OSF-2 DuoSet ELISA Kit (Catalog # DY3548B) for convenient development of a sandwich ELISA.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Periostin/OSF-2
References
- Liu, A.Y. et al. (2014) Matrix Biol. 37:150.
- Gillan, L. et al. (2002) Cancer Res. 62:5358.
- Takeshita, S. et al. (1993) Biochem. J. 294:271.
- Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
- Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
- Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
- Li, G. et al. (2006) Atherosclerosis 188:292.
- Shao, R. et al. (2004) Mol. Cell. Biol. 24:3992.
- Yan, W. and R. Shao (2006) J. Biol. Chem. 281:19700.
- Erkan, M. et al. (2007) Gastroenterology 132:1447.
- Norris, R.A. et al. (2007) J. Cell. Biochem. 101:695.
- Kudo, Y. et al. (2012) PLoS One 7:e44488.
- Snider, P. et al. (2008) Circ. Res. 102:752.
- Kuhn, B. et al. (2007) Nat. Med. 13:962.
- Blanchard, C. et al. (2008) Mucosal Immunol. 1:289.
- Sehra, S. et al. (2011) J. Immunol. 186:4959.
Long Name
Osteoblast Specific Factor 2
Alternate Names
Fasciclin I-like, OSF-2, OSF2, POSTN, TRIF52
Gene Symbol
POSTN
UniProt
Additional Periostin/OSF-2 Products
Product Documents for Human Periostin/OSF-2 Antibody
Product Specific Notices for Human Periostin/OSF-2 Antibody
For research use only
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