Human Peroxiredoxin 6 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3490
Key Product Details
Species Reactivity
Validated:
Human
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Clone # 477068
Product Summary for Human Peroxiredoxin 6 Antibody
Immunogen
E. coli-derived recombinant human Peroxiredoxin 6
Met1-Pro224
Accession # P30041
Met1-Pro224
Accession # P30041
Specificity
Detects endogenous human Prx-6 in Western blots. In Western blots, this antibody does not cross-react with rhPrx-1, -2, -3, -4, or -5.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Scientific Data Images for Human Peroxiredoxin 6 Antibody
Detection of Human Peroxiredoxin 6 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Peroxiredoxin 6 Monoclonal Antibody (Catalog # MAB3490) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Peroxiredoxin 6 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human Peroxiredoxin 6 by Simple WesternTM.
Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Peroxiredoxin 6 at approximately 31 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Peroxiredoxin 6 Monoclonal Antibody (Catalog # MAB3490) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Peroxiredoxin 6 Antibody
Application
Recommended Usage
Simple Western
5 µg/mL
Sample: HEK293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line
Sample: HEK293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: HEK293 human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line
Sample: HEK293 human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 3.5 using MAB3490 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Peroxiredoxin 6
Human Peroxiredoxin 6 (Prx-6) is a 26 kDa, cytosolic antioxidant enzyme that belongs to the 1-Cys class of the THP/ahpC family of proteins. This protein is 224 amino acids (aa) in length and has one catalytic cysteine at Cys46. Following an attack on peroxide, Cys46 is oxidized to cysteine sulfenic acid and exists in a stable conformation. Reduced, Prx-6 is a homotetramer. When activated, it apparently forms a covalently-associated homodimer. In addition to glutathione peroxidase activity, Prx-6 is also reported to demonstrate phospholipase A2 activity. Thus, Prx-6 likely plays a role in phospholipid turnover. Ser32 in a GWSWG motif is required for its enzymatic activity. Human Prx-6 is 92% and 90% aa identical to rat and mouse Prx-6, respectively.
Alternate Names
aiPLA2, AOP2, NSGPx, PRDX6
Gene Symbol
PRDX6
UniProt
Additional Peroxiredoxin 6 Products
Product Documents for Human Peroxiredoxin 6 Antibody
Product Specific Notices for Human Peroxiredoxin 6 Antibody
For research use only
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