Human Phospho-BTK (Y551) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7659
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 797837
Product Specifications
Immunogen
Phosphopeptide containing the human BTK Y551 site
Specificity
Detects human BTK when phosphorylated at Y551.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Phospho-BTK (Y551) Antibody
Detection of Human Phospho-BTK (Y551) by Western Blot.
Western blot shows lysates of RL human non-Hodgkin's lymphoma B cell line and Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 0.1 mM Pervanadate (PV) for 10 minutes and 12 µg/mL Goat Anti-Human IgM µ Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # G-105-C) for 2 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-BTK (Y551) Monoclonal Antibody (Catalog # MAB7659) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-BTK (Y551) at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Phospho-BTK (Y551) by Simple WesternTM.
Simple Western lane view shows lysates of Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with ultraviolet light (UV), loaded at 0.2 mg/mL. A specific band was detected for Phospho-BTK (Y551) at approximately 89 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Phospho-BTK (Y551) Monoclonal Antibody (Catalog # MAB7659). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Phospho-BTK (Y551) Antibody
Application
Recommended Usage
Simple Western
20 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line treated with ultraviolet light (UV)
Sample: Ramos human Burkitt's lymphoma cell line treated with ultraviolet light (UV)
Western Blot
1 µg/mL
Sample: RL human non-Hodgkin's lymphoma B cell line and Ramos human Burkitt's lymphoma cell line treated (+) Pervanadate (PV) and Goat Anti-Human IgM μ Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # G-105-C) for 2 minutes
Sample: RL human non-Hodgkin's lymphoma B cell line and Ramos human Burkitt's lymphoma cell line treated (+) Pervanadate (PV) and Goat Anti-Human IgM μ Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # G-105-C) for 2 minutes
Reviewed Applications
Read 1 review rated 5 using MAB7659 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BTK
Long Name
Bruton Agammaglobulinemia Tyrosine Kinase
Alternate Names
AGMX1, IMD1, PSCTK1, XLA
Entrez Gene IDs
695 (Human)
Gene Symbol
BTK
Additional BTK Products
Product Documents for Human Phospho-BTK (Y551) Antibody
Product Specific Notices for Human Phospho-BTK (Y551) Antibody
For research use only
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