Human Phospho-Chk2 (T68) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1626
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1238H
Product Specifications
Immunogen
E.coli-derived recombinant human Phospho-Chk2 (T68)
Accession # O96017
Accession # O96017
Specificity
Detects human Chk2 when phosphorylated at T68 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-Chk2 (T68) Antibody
Detection of Human Chk2 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 5 hours. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human Phospho-Chk2 (T68) Monoclonal Antibody (Catalog # MAB1626) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Chk2 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-Chk2 (T68) in HeLa Human Cell Line.
Chk2 phosphorylated at T68 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line unstimulated (lower panel) or stimulated with 1 µM Camptothecin (upper panel) using Rabbit Anti-Human Phospho-Chk2 (T68) Monoclonal Antibody (Catalog # MAB1626) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei (upper panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Phospho-Chk2 (T68) Antibody
Application
Recommended Usage
Immunocytochemistry
2-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line stimulated with 1 μM Camptothecin
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line stimulated with 1 μM Camptothecin
Western Blot
2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with Camptothecin (CPT)
Sample: HeLa human cervical epithelial carcinoma cell line treated with Camptothecin (CPT)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chk2
Serine/threonine-protein kinase Chk2 (CHEK2) is a 65 kDa member of Serine/threonine-protein kinase family of proteins. Chk2 regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Chk2 is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks.Existence of multiple splice variants of Chk2 is predicted. Human Chk2 shares 86% aa sequence identity with mouse Chk2.
Long Name
Checkpoint Kinase 2
Alternate Names
CDS1, CHEK2, HuCds1, LFS2, PP1425, Rad53
Gene Symbol
CHEK2
UniProt
Additional Chk2 Products
Product Documents for Human Phospho-Chk2 (T68) Antibody
Product Specific Notices for Human Phospho-Chk2 (T68) Antibody
For research use only
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