May I know which creb1 antibody is good for ChIP? I found a paper using your rabbit polyclonal creb1 antibody for ChIP but they didn't indicate the catalog number.

Name: Human Phospho-CREB (S133) Antibody
Brand: R&D Systems
SKU: MAB6906
Rating: 4 (4 reviews)

We are not aware of any of our Creb1 antibodies being used in ChIP at this time. If you can provide us with the PMID of the paper you are referring to I can contact the author. Otherwise, you can use our Innovators Reward Program to try an antibody in a novel species and application.

Human Phospho-CREB (S133) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6906

(4)

Product Datasheet / COA / SDS

100% Guarantee

Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # 702710

View all CREB Antibodies »

Product Summary for Human Phospho-CREB (S133) Antibody

Immunogen

Phosphopeptide containing the human CREB S133 site

Specificity

Detects human CREB when phosphorylated at S133.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human Phospho-CREB (S133) Antibody

Detection of Human Phospho-CREB (S133) by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HEK293 human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm²ultraviolet light (UV) with a 30 minute recovery. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-CREB (S133) Monoclonal Antibody (Catalog # MAB6906) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-CREB (S133) at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Phospho-CREB (S133) in HeLa Human Cell Line.

CREB phosphorylated at A133 was detected in immersion fixed Hela human cervical epithelial carcinoma cells, unstimulated (lower panel) and stimulated (upper panel) with PMA, using Mouse Anti-Human Phospho-CREB (S133) Monoclonal Antibody (Catalog # MAB6906) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Phospho-CREB (S133) Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Hela human cervical epithelial carcinoma cells stimulated with PMA

Western Blot

0.5 µg/mL
Sample: Hela human cervical epithelial carcinoma cells and HEK293 human embryonic kidney cells stimulated with ultraviolet light (UV).

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4 using MAB6906 in the following applications:

Immunocytochemistry/Immunofluorescence (2 Reviews)
Western Blot (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CREB

The cAMP response element binding protein (CREB) belongs to the bZIP superfamily of transcription factors, containing a basic domain that mediates DNA binding and a leucine zipper domain that facilitates dimerization. Within the promoter of target genes, CREB dimers bind cAMP response elements, defined by the palindromic consensus sequence TGACGTCA. When phosphorylated at Ser133, CREB also binds the coactivator CREB binding protein (CBP), which enhances transcription by acetylating histones to facilitate chromatin unraveling.

Long Name

cAMP Response Element-binding Protein

Alternate Names

CREB1

Entrez Gene IDs

1385 (Human); 12912 (Mouse)

Gene Symbol