Human Phospho-ER alpha/NR3A1 (S118) Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5954
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Phosphopeptide containing the human ER alpha/NR3A1 S118 site
Accession # P03372
Accession # P03372
Specificity
Detects human Phospho-ER alpha/NR3A1 (S118) in Western blots.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-ER alpha/NR3A1 (S118) Antibody
Detection of Human Phospho‑ER alpha/NR3A1 (S118) by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 nM estradiol for 60 minutes. PVDF Membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-ERa/NR3A1 (S118) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5954) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-ERa/NR3A1 (S118) at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human Phospho-ER alpha/NR3A1 (S118) Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: MCF‑7 human breast cancer cell line treated with estradiol
Sample: MCF‑7 human breast cancer cell line treated with estradiol
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ER alpha/NR3A1
ER alpha (Estrogen receptor alpha; also Estradiol receptor and NR3A1) is a 65‑70 kDa member of the NR3 subfamily, nuclear hormone receptor family of proteins. It is widely expressed, and serves as a strong activator of estrogen-responsive genes. ER alpha is normally quiescent and bound to heat-shock proteins and immunophilins. Following beta-estradiol binding, it becomes activated, either homodimerizes or heterodimerizes with ER beta, and binds to DNA with multiple co-activators. Major phosphorylation sites exist at Tyr537, Ser167 and Ser118. Phosphorylation at Ser118 regulates ER alpha's role in transcription and cell growth.
Long Name
Estrogen Receptor alpha
Alternate Names
ESR1, NR3A1
Entrez Gene IDs
2099 (Human)
Gene Symbol
ESR1
UniProt
Additional ER alpha/NR3A1 Products
Product Documents for Human Phospho-ER alpha/NR3A1 (S118) Antibody
Product Specific Notices for Human Phospho-ER alpha/NR3A1 (S118) Antibody
For research use only
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