Human Phospho-FADD (S194) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7047
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 709736
Product Specifications
Immunogen
Phosphopeptide containing the human FADD S194 site
Specificity
Detects human FADD when phosphorylated on Ser194.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Phospho-FADD (S194) Antibody
Detection of Human Phospho-FADD (S194) by Western Blot.
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µg/mL nocodazole or 4 mM hydroxyurea for 20 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-FADD (S194) Monoclonal Antibody (Catalog # MAB7047) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FADD at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-FADD (S194) in HeLa Human Cell Line.
FADD phosphorylated at S194 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line stimulated with nocadazole using Mouse Anti-Human Phospho-FADD (S194) Monoclonal Antibody (Catalog # MAB7047) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Phospho-FADD (S194) Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line stimulated with nocadazole
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line stimulated with nocadazole
Western Blot
1 µg/mL
Sample: HT‑29 human colon adenocarcinoma cell line and HeLa human cervical epithelial carcinoma cell line treated with nocodazole or hydroxyurea for 20 hours
Sample: HT‑29 human colon adenocarcinoma cell line and HeLa human cervical epithelial carcinoma cell line treated with nocodazole or hydroxyurea for 20 hours
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FADD
Long Name
Fas-associated Death Domain Protein
Alternate Names
MORT1
Entrez Gene IDs
8772 (Human)
Gene Symbol
FADD
Additional FADD Products
Product Documents for Human Phospho-FADD (S194) Antibody
Product Specific Notices for Human Phospho-FADD (S194) Antibody
For research use only
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