Human Phospho-FGFR1-4 (Y653/Y654) Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3285
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Phosphopeptide containing human FGF R1 Y653/Y654 sites
Specificity
Detects human FGF R when dually phosphorylated at Y653/Y654 in Western blots.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-FGFR1-4 (Y653/Y654) Antibody
Detection of Human Phospho- FGF R1‑4 (Y653/Y654) by Western Blot.
Western blot shows lysates of KATO-III human gastric carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recom-binant Human FGF acidic (Catalog # 232-FA) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-FGF R1-4 (Y653/Y654) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3285), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-FGF R1-4 (Y653/Y654) at approximately 120 to 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-FGF R1-4 (Y653/Y654) in A431 Human Cell Line.
FGF R1-4 phosphorylated at Y653/Y654 was detected in immersion fixed A431 human epithelial carcinoma cell line untreated (lower panel) or treated (upper panel) with pervanadate using Rabbit Anti-Human Phospho-FGF R1-4 (Y653/Y654) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3285) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Phospho-FGFR1-4 (Y653/Y654) Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line treated with pervanadate
Sample: Immersion fixed A431 human epithelial carcinoma cell line treated with pervanadate
Western Blot
0.5 µg/mL
Sample: KATO-III human gastric carcinoma cell line treated with Recombinant Human FGF acidic (Catalog # 232-FA)
Sample: KATO-III human gastric carcinoma cell line treated with Recombinant Human FGF acidic (Catalog # 232-FA)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGFR1-4
Long Name
Fibroblast Growth Factor Receptors 1-4
Alternate Names
BFGFR;bFGF-R-1;CD331;CEK;ECCL;FGFBR;FGFR-1;Fibroblast growth factor receptor;Fibroblast growth factor receptor 1;FLG;FLT2;FLT-2;HBGFR;HH2;HRTFDS;KAL2;N-SAM;OGD
Additional FGFR1-4 Products
Product Documents for Human Phospho-FGFR1-4 (Y653/Y654) Antibody
Product Specific Notices for Human Phospho-FGFR1-4 (Y653/Y654) Antibody
For research use only
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