Human Phospho-GSK-3 beta (S9) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8934
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunocytochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1253C
Product Specifications
Immunogen
Phosphopeptide containing the human GSK‑3 beta S9 site
Specificity
Detects human GSK‑3 beta when phosphorylated at S9 in Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-GSK-3 beta (S9) Antibody
Detection of Human Phospho-GSK‑3 beta (S9) by Western Blot.
Western blot shows lysates of untreated (-) or MCF-7 human breast cancer cell line treated (+) with 100 ng/ml Recombinant Human IGF-I (Catalog # 291-G1) for 60 minutes and HeLa human cervical epithelial carcinoma cell line treated (+) with 200 nM PMA for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-GSK-3 beta Monoclonal Antibody (Catalog # MAB8934) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-GSK-3 beta (S9) at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-GSK-3 beta (S9) in MCF‑7 Human Cell Line.
GSK-3 beta phosphorylated at S9 was detected in immersion fixed MCF-7 human breast cancer cell line untreated (lower panel) or treated with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1; upper panel) using Rabbit Anti-Human Phospho-GSK-3 beta Monoclonal Antibody (Catalog # MAB8934) at a 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Phospho-GSK‑3 beta (S9) by Simple WesternTM.
Simple Western lane view shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1) for 60 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-GSK-3 beta (S9) at approximately 56 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Phospho-GSK-3 beta Monoclonal Antibody (Catalog # MAB8934) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.Applications for Human Phospho-GSK-3 beta (S9) Antibody
Application
Recommended Usage
Immunocytochemistry
1-25 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line treated with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1)
Sample: Immersion fixed MCF‑7 human breast cancer cell line treated with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1)
Simple Western
5 µg/mL
Sample: MCF‑7 human breast cancer cell line treated with Recombinant Human IGF‑I (Catalog # 291-G1)
Sample: MCF‑7 human breast cancer cell line treated with Recombinant Human IGF‑I (Catalog # 291-G1)
Western Blot
0.5 µg/mL
Sample: MCF‑7 human breast cancer cell line treated with Recombinant Human IGF‑I (Catalog # 291-G1) and HeLa human cervical epithelial carcinoma cell line treated with PMA
Sample: MCF‑7 human breast cancer cell line treated with Recombinant Human IGF‑I (Catalog # 291-G1) and HeLa human cervical epithelial carcinoma cell line treated with PMA
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GSK-3 beta
Long Name
Glycogen Synthase Kinase 3 beta
Alternate Names
GSK3 beta, GSK3B
Gene Symbol
GSK3B
Additional GSK-3 beta Products
Product Documents for Human Phospho-GSK-3 beta (S9) Antibody
Product Specific Notices for Human Phospho-GSK-3 beta (S9) Antibody
For research use only
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