Human Phospho-MEK1 (T386) Antibody
R&D Systems, part of Bio-Techne | Catalog # PPS076
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Summary for Human Phospho-MEK1 (T386) Antibody
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the human phospho-T386 of MEK1
Specificity
Human ~45 kDa MEK1 phosphorylated at T386
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-MEK1 (T386) Antibody
Detection of Phospho-MEK1 (T386) by Western Blot
Western blot of human T47D cells showing specific immunolabeling of the ~45 kDa MEK1 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda phosphatase, lambda PPase). The blot is identical to the control except that it was incubated in lambda PPase (1200 units for 30 minutes) before being exposed to the MEK1 (T386) antibody. The immunolabeling of MEK1 is completely eliminated by lambda PPase.Applications for Human Phospho-MEK1 (T386) Antibody
Application
Recommended Usage
Western Blot
1:1000 dilution
Sample: T47D human breast cancer cell line
Sample: T47D human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: MEK1
References
- Chong, H. et al. (2003) Cell. Signal. 15:463.
- Eblen, S.T. et al. (2004) Mol. Cell. Biol. 24:2308.
- Xu, B. et al. (1999) J. Biol. Chem. 274:34029.
- Frost, J.A. et al. (1997) EMBO J. 16:6426.
- Mansour, S.J. et al. (1994) J. Biochem. 116:304.
- Zheng, C-F. and K-L. Guan (1994) EMBO J. 13:1123.
Long Name
Mitogen-activated Protein Kinase Kinase 1
Alternate Names
MAP2K1, MKK1
Gene Symbol
MAP2K1
Additional MEK1 Products
Product Documents for Human Phospho-MEK1 (T386) Antibody
Product Specific Notices for Human Phospho-MEK1 (T386) Antibody
For research use only
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