Human Phospho-MLKL (T357) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9187
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 954702
Product Specifications
Immunogen
Phosphopeptide containing the human MLKL T357 site
Accession # Q8NB16
Accession # Q8NB16
Specificity
Detects human MLKL when phosphorylated at T357.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Phospho-MLKL (T357) Antibody
Phospho-MLKL (T357) in Human Squamous Cell Carcinoma.
MLKL phosphorylated at T357 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human Phospho-MLKL (T357) Monoclonal Antibody (Catalog # MAB9187) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human Phospho-MLKL (T357) Antibody
Application
Recommended Usage
Immunohistochemistry
1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human squamous cell carcinoma
Sample: Immersion fixed paraffin-embedded sections of human squamous cell carcinoma
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MLKL
Long Name
Mixed Lineage Kinase Domain-like
Alternate Names
FLJ34389, mixed lineage kinase domain-like, mixed lineage kinase domain-like protein
Gene Symbol
MLKL
UniProt
Additional MLKL Products
Product Documents for Human Phospho-MLKL (T357) Antibody
Product Specific Notices for Human Phospho-MLKL (T357) Antibody
For research use only
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