Human Phospho-p53 (S46) Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1489
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Phosphopeptide containing human p53 S46 site
Specificity
Detects human p53 when phosphorylated at S46.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-p53 (S46) Antibody
Detection of Human Phospho-p53 (S46) by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 5 hours. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-p53 (S46) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1489) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p53 (S46) was detected at approximately 53 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 30 minutes. For additional reference the membrane was stripped and reprobed for total p53 using Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # HAF1355). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
p53 in Human Breast Cancer Tissue.
p53 phosphorylated at S46 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human Phospho-p53 (S46) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1489) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
p53 in Human Breast.
p53 phosphorylated at S46 was detected in immersion fixed paraffin-embedded sections of human breast array using Rabbit Anti-Human Phospho-p53 (S46) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1489) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Phospho-p53 (S46) Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue and normal human breast array
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue and normal human breast array
Simple Western
10 µg/mL
Sample: MCF‑7 human breast cancer cell line treated with Camptothecin (CPT)
Sample: MCF‑7 human breast cancer cell line treated with Camptothecin (CPT)
Western Blot
1 µg/mL
Sample: MCF-7 human breast cancer cell line treated with camptothecin
Sample: MCF-7 human breast cancer cell line treated with camptothecin
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p53
The p53 tumor suppressor protein signals apoptosis in cells that have incurred irreparable genotoxic damage. Phosphorylation of p53 at serine 46 is a critical event leading to the cellular decision to undergo apoptosis rather than cell cycle arrest. Serine 46 can be phosphorylated by either the p38 MAP kinase or the homeodomain-interacting protein kinase 2 (HIPK2).
Alternate Names
BCC7, LFS1, TP53, TRP53
Gene Symbol
TP53
Additional p53 Products
Product Documents for Human Phospho-p53 (S46) Antibody
Product Specific Notices for Human Phospho-p53 (S46) Antibody
For research use only
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