Human Phospho-PLC-gamma 2 (Y753) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB37161
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 790623
Product Summary for Human Phospho-PLC-gamma 2 (Y753) Antibody
Immunogen
Phosphopeptide containing the human PLC‑ gamma2 Y753 site
Accession # P16885
Accession # P16885
Specificity
Detects human PLC‑ gamma2 when phosphorylated at Y753.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Phospho-PLC-gamma 2 (Y753) Antibody
Detection of Human Phospho-PLC‑ gamma2 (Y753) by Western Blot.
Western blot shows lysates of RL human non-Hodgkin's lymphoma B cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-PLC- gamma2 (Y753) Monoclonal Antibody (Catalog # MAB37161) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-PLC- gamma2 (Y753) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-PLC-gamma 2 (Y753) Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: RL human non-Hodgkin's lymphoma B cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line treated with Pervanadate
Sample: RL human non-Hodgkin's lymphoma B cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line treated with Pervanadate
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLC-gamma 2
The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-delta, -beta, -gamma, -epsilon, -zeta, and -eta. PLC-gamma 2 (Phospholipase C gamma-2) contains 2 SH2 and 1 SH3 domains and is primarily limited to cells of hematopoietic lineage. PLC-gamma 2 is activated by receptor tyrosine kinases in response to growth factors, neurotransmitters, and hormones, and downstream through Lck kinase-dependent phosphorylation at Y753 and Y759. Activated PLC-gamma 2 catalyzes the hydrolysis of phosphatidylinositol 4, 5-biphosphate to produce the second messengers inositol 1, 4, 5-triphosphate (IP3) and diacylglycerol (DAG). IP3 mobilizes the release of calcium while DAG activates protein kinase C. PLC-gamma 2 is involved in collagen induced signaling in platelets and antigen-dependent signaling in B-lymphocytes. Human PLC-gamma 2 shares 94% and 95% sequence identity overall with mouse and rat PLC-gamma 2, respectively, and 100% sequence identity within the peptide immunogen.
Long Name
Phospholipase C gamma 2
Alternate Names
PLCG2, PLCgamma 2
Gene Symbol
PLCG2
UniProt
Additional PLC-gamma 2 Products
Product Documents for Human Phospho-PLC-gamma 2 (Y753) Antibody
Product Specific Notices for Human Phospho-PLC-gamma 2 (Y753) Antibody
For research use only
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