Human Phospho-RelA/NFkB p65 (S529) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7624
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 795217
Product Specifications
Immunogen
Phosphopeptide containing the human RelA/NF kappaB p65 S529 site
Accession # Q04206
Accession # Q04206
Specificity
Detects human RelA/NF kappaB p65 when phosphorylated at S529
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Phospho-RelA/NFkB p65 (S529) Antibody
Detection of Human Phospho-RelA/NF kappaB p65 (S529) by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-RelA/NF kappa B p65 (S529) Monoclonal Antibody (Catalog # MAB7624) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-RelA/NF kappa B p65 (S529) at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-RelA/NFkB p65 (S529) Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Sample: HeLa human cervical epithelial carcinoma cell line treated Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RelA/NFkB p65
RelA belongs to a family of transcription factors (NF kappaB (nuclear factor kappa from B cells) complex) that play a fundamental role in inflammatory and immune responses. The NF kappaB complex is composed of a heterodimer of a Rel family member (RelA, c-Rel, RelB) and either NF kappaB1 or NF kappaB2 subunits. RelA and NF kappaB1 are the most common heterodimeric pair. The NF kappaB complex is sequestered in the cytoplasm by inhibitory I kappaB proteins. Upon cellular activation, the ubiquitin-proteosome pathway degrades the I kappaB proteins allowing the NF kappaB complex to translocate to the nucleus and activate gene transcription.
Long Name
v-rel Reticuloendotheliosis Viral Oncogene Homolog A
Alternate Names
NFkB p65, NFKB3, p65RelA
Entrez Gene IDs
5970 (Human)
Gene Symbol
RELA
UniProt
Additional RelA/NFkB p65 Products
Product Documents for Human Phospho-RelA/NFkB p65 (S529) Antibody
Product Specific Notices for Human Phospho-RelA/NFkB p65 (S529) Antibody
For research use only
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